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Improvement of experimentally-phased maps

IF5A MAD map

(See: Peat, T. S., Newman, J., Waldo, G. S., Berendzen, J. & Terwilliger, T. C. (1998) "Structure of translation initiation factor 5A from Pyrobaculum aerophilum at 1.75 A resolution" Structure 15, 1207-1214.

MAD dataset on initiation factor 5A

Only 1 of 3 selenium atoms in 147 residues used in phasing (just for this test example)

Solvent content was 60%

Resolution of 2 A, space group I4

Initial correlation of experimental map with one based on refined model was 0.37

Correlation after RESOLVE was 0.79

Correlation after conventional solvent flattening and histogram matching (using dm) was 0.65

solve bright

Initial MAD map (CC=0.37)

resolve bright 2

RESOLVE map (CC=0.79)


Conventional solvent flattening & histogram matching (CC=0.65)

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