What do I do if Resolve says:

• Sorry, the protein region has no volume? This means that the solvent content is 0 or 1. Try a value in between.
• Sorry, this is an invalid access code You need to check out your solve2.access file. If SOLVE runs, so should Resolve.
• Segmentation fault (core dump, program never starts) On an HP or an SGI: This happens if your machine doesn't have enough memory allocated to you. To get around this (at least on an SGI), use the command: "unlimit" . You can put this as one line in your .cshrc file if you use csh. The same works on an Alpha too.
• killed This happens if you run the too big a version of resolve on an SGI or if you're already running a big job. See the above command "unlimit" to fix it
• /sbin/loader: Fatal Error: Program datasize exceeds process datasize limit. This is an error message you get with an Alpha when you don't have enough memory allocated to yourself. To get around this, edit your ".cshrc" or similar file to include the line "unlimit".
• Sorry, nothing in bins of density? The electron density map you input probably has no reflections in the resolution range or is all zeros.
• Sorry, cannot add any more onto the "LABIN" line (max 132 characters). Resolve cannot use more than 132 characters in a labin line. Sorry!
• Sorry: uninterpretable value or keyword. Resolve could not parse the input. It stops when this happens. Usually a keyword it does not recognize was input. Note that resolve does not recognize partial keywords, you have to type the whole word in.
• Sorry, need to make n_map_max at least [some number]. The version of Resolve you have is not big enough for your dataset. Try the "giant" (resolve_giant) or even the "huge" (resolve_huge) version. If that does not work, contact the author for a bigger version.
• sorry, this map has no density. The electron density map you input probably has no reflections in the resolution range or is all zeros.
• Sorry, need to increase n_refl_max The version of Resolve you have is not big enough for your dataset. Try the giant or huge versions (resolve_giant or resolve_huge). If that does not work, contact the author for a bigger version.
• rms of this map is nan avg = nan This can happen if there are "NaN" (empty) entries in your mtz file. This version of resolve does not always handle these correctly. If you remove them (mtzdump, remove lines with question marks, f2mtz) it should be ok.
• resolve: Open failed: File: SYMOP. Resolve needs the CCP4 symmetry data file "symop.lib". Usually this is located in /usr/local/lib/resolve after you download resolve. If it is not there, then you'll need to find out where it is, then define the environmental variable SYMOP with: setenv SYMOP /usr/local/lib/resolve/symop.lib [or wherever it is].

 

• Extend phases or fill in missing reflections?  Yes, any reflections with non-zero F in your input file that are in your resolution limits are filled in by resolve (version 1.04 or higher).
• Use NC-symmetry?  Yes, starting with version 2.0.
• Phase from a partial model?  Yes, use prime-and-switch phasing for this one
• Use a MR starting model?  Yes, use prime-and-switch for this too