# command file to solve a 2-deriv MIR dataset using ccp4 mtz input # It looks just like one_mir_dataset.html except it has LABIN and HKLIN # and it has no rawnativefile or rawderivfile and no scale_native or # scale_derivative # CCP4_OPEN environmental variable set to UNKNOWN so file overwriting will work setenv CCP4_OPEN UNKNOWN solve <<EOD ! ccp4 mtz file input ! solve a 2-deriv MIR dataset logfile mir.logfile ! write out most information to this file. ! summary info will be written to solve.prt resolution 20 3 ! you need resolution. space group and cell ! dimensions read from mtz file. ! get the mtz file information and read it in: labin FP=FP SIGFP=SIGFP FPH1=FPH1 SIGFPH1=SIGFPH1 DPH1=DPH1 SIGDPH1=SIGDPH1 labin FPH2=FPH2 SIGFPH2=SIGFPH2 DPH2=DPH2 SIGDPH2=SIGDPH2 hklin mir_fbar-cad.mtz ! now we're ready with scaled data derivative 1 ! about to enter information on derivative #1 label deriv 1 HG ! a label for this deriv atomname hg inano ! You need to tell it if anomalous diffs are ! to be used noanorefine ! use anomalous differences in phasing ! but not refinement (best option for MIR) nsolsite_deriv 2 ! max 2 sites this deriv derivative 2 label deriv 2 also hg atomname hg inano noanorefine ! use anomalous differences in phasing ! but not refinement (best option for MIR) acceptance 0.35 ! accept a new site if it has a ! peak height about 1/3 of avg or more nsolsite 2 ! number of sites per deriv ! (use nsolsite_deriv to set individual values) ANALYZE_MIR ! analyze this mir data and set up for SOLVE SOLVE EOD